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KMID : 1094720170220050504
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Biotechnology and Bioprocess Engineering
2017 Volume.22 No. 5 p.504 ~ p.511
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Evaluating the role of puckering and fluorine atom in stability and folding of fluoroproline containing proteins
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Nadarajan Saravanan Prabhu
Deepankumar Kanagavel
Seo Joo-Hyun
Yun Hyung-Don
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Abstract
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In the past decade, numerous studies have been reported that the residue specific incorporation of fluorine containing analogs into protein can enhance the stability of protein. On the other hand, the incorporation of fluoroproline can enhance both stability and refolding rate of recombinant proteins. The objective of this study was to determine the reason behind the enhanced stability and refolding rate of protein by comparing GFP variants containing fluoroproline or hydroxyproline. The fluorine atom of 4-fluoroproline played a significant role in enhancing stability, and Cγ-endo puckering property of (4S)-4-fluoroproline and (4S)-4-hydroxyproline plays a key role in enhancing protein refolding rate.
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KEYWORD
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enzyme stabilization, fluorination, fluoroproline, protein folding, unnatural amino acid
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